After obtaining a BSC in Animal Biology at the University of Yaoundé1, Dr Magellan TCHOUAKUI conducted a master research at the same university on the topic ‘’Assessing the impact of ivermectin and albendazole mass treatments administration on the transmission of lymphatic filariasis in the North and Far North Cameroon’’, defended in December 2014. During his master carrier, He conducted a Monitoring and Evaluation of baseline data for lymphatic filariasis, onchocerciasis, loasis, malaria and geohelmenthiasis in the Eastern region of Cameroon and Testing of efficacy and feasibility of the Loa Cellscope for the control of Onchocerciasis in the area where both loasis and onchocerciasis are co-endemic: Okola (Centre Cameroon).
Curled from a pool of Ph.D students being mentored at the Centre for Research in Infectious Diseases (CRID), Dr Magellan TCHOUAKUI emerges first Ph.D now a Doctor from CRID. He successfully defended his PhD on June 16, 2020 on the topic “Fitness cost of metabolic resistance to insecticide in the major African malaria vector Anopheles funestus”, a research work which took off in October 2015. He conducted he’s Ph.D at the University of Yaoundé I under the supervision of Professor of Genetics at the Liverpool School of Tropical Medicine Prof Charles Wondji and Lecturer at the University of Yaoundé I Prof. Flobert NJIOKOU. During his PhD, Dr Magellan Tchouakui has been mostly implicated in :
-Field collection, morphological and molecular identification of malaria vectors (AN. FUNESTUS and AN. GAMBIAE) across Cameroon (Simatou, Far North; Gounougou, North; Tibati and Mibellon, Adamawa; Bankeng and Elon, Center; Obout, south Cameroon), in Southern Tanzania (Ifakara) in Chad (NDjamena and Massacory) and Uganda (Tororo, Busia and Mayuge) using Electric aspirators and HLC method for adult collection and dipping method for larval collection;
– Rearing of field collected malaria mosquitoes (AN. GAMBIAE and AN. FUNESTUS) and lab strain mosquitoes (FUMOZ, FANG and KISUMU strains, etc) in the insectary;
– Mosquitoes ovary dissection
– Characterisation of resistance status of malaria vectors using WHO procedures against all classes of insecticide used in public health: pyrethroids, organochlorines, carbamates, organophosphates. These procedures include cone assays with various impregnated mosquito nets, WHO tubes assays, CDC bottle assay, etc;
– Identification, Mapping and Collection of ANOPHELES FUNESTUS swarms in Cameroon and Southern Tanzania for the study of mating competitiveness;
– Assessing of the fitness cost of metabolic and target site resistance mechanisms on AN. FUNESTUS field mosquitoes by genotyping of sevral resistance mechanisms using various technics (AS-PCR, PCR-RFLP, Lamp PCR, Taqman, RT-qPCR);
– Analysis of genetic diversity and gene flow in vector populations
– Contribution in testing the new insecticides from IVCC and evaluation of chlorfenapyr by CDC bottle Assay Method
– Longitudinal entomological surveillance of Malaria vectors in the Far-North Cameroon under the PMI (President Malaria Initiative) / Vectorlink project
- Areas of interest:
Malaria & Insecticide resistance management
- Research – an overview of his research areas(s)
In pursuit of his career as researcher, Dr Magellan TCHOUAKUI continues as a Post-doctoral Research Assistant at CRID working in the Senior Renewal Fellowship project of Prof Charles Wondji. This project aims at Elucidating the complex evolution of insecticide resistance in the major malaria vector Anopheles funestus Africa-wide and its impact on control programmes. He focuses his research mainly on selecting and elucidating the molecular basis of resistance to new WHO-recomended insecticides.
- Research Areas:
I am a member of Wondji’s research unit (WoRU)
- PhD Students: Dr Magellan TCHOUAKUI contributes in the training of young scientist and is mentoring a group of about 10 Ph.D and Master’s students
- Tepa Arnaud,
- Tatiane Assatse,
- Tazokong Hervé,
- Mahamat Gadji,
- Fleuriane Metissa,
- Lamo Sonia,
- Natchema Fonkou Brice,
- Ricardo Thiomela
Master students: Derrick Fofié, Claudine Raissa Tientcheu
Other relevant expertise, professional memberships, etc
- 2018-now: Member of Pan-African Mosquitoes Association (PAMCA)
- 2018-now: Member of the US-UK Collab: RCN: Vector Behavior in Transmission Ecology (VectorBiTE) Network
- 2018-now: Member of Antivec, BOVA and CONNECTED Networks
Dr Magellan is the author of about 20 scientific publications in international journals including Science Translational Medicine, Nature communications, Nature Scientific reports, Nature Heredity… To read his scientific publications
https://www.researchgate.net/profile/Magellan_Tchouakui2 and http://orcid.org/0000-0002-1683-8057
– TCHOUAKUI, M., MIRANDA, J. R., MUGENZI, L. M., DJONABAYE, D., WONDJI, M. J., TCHOUPO, M., et al. 2020. Cytochrome P450 metabolic resistance (CYP6P9a) to pyrethroids imposes a fitness cost in the major African malaria vector Anopheles funestus. Heredity, 124, 621-632.
– Mugenzi, LMJ, Menze, BD,Tchouakui, M, et al. A 6.5‐kb intergenic structural variation enhances P450‐mediated resistance to pyrethroids in malaria vectors lowering bed net efficacy. Mol Ecol 2020; 00: 1– 17. https://doi.org/10.1111/mec. 15645
– Nouage L, Elanga-Ndille E, Binyang A, Tchouakui M, Atsatse T, Ndo C, Kekeunou S, Wondji CS. Influence of GST- and P450-based metabolic resistance to pyrethroids on blood feeding in the major African malaria vector Anopheles funestus. PLoS One. 2020 Sep 18;15(9):e0230984. doi: 10.1371/journal.pone. 0230984
– Djuicy, D.D.; Hearn, J.; Tchouakui, M.; Wondji, M.J.; Irving, H.; Okumu, F.O.; Wondji, C.S. CYP6P9-Driven Signatures of Selective Sweep of Metabolic Resistance to Pyrethroids in the Malaria Vector Anopheles funestus Reveal Contemporary Barriers to Gene Flow. Genes 2020, 11, 1314.
– Elanga-Ndille, E., Binyang, A., Ndo, C., Assatse, T., Nouage, L., Tchouakui, M., … & Wondji, C. S. (2020). Entomological indicators of malaria transmission and insecticide resistance profile of Anopheles gambiae at the early phase of irrigated rice farming in the forest area of central Cameroon. Wellcome Open Research, 5(19 0), 190.
– Gueye, O., Tchouakui, M., Dia, A. K., Faye, M. B., Ahmed, A. A., Wondji, M. J., … & Wondji, C. S. (2020). Insecticide Resistance Profiling of Anopheles coluzzii and Anopheles gambiae Populations in the Southern Senegal: Role of Target Sites and Metabolic Resistance Mechanisms. Genes, 11(12), 1403.