Insectary

Our biosafety level 2 insectary provides a high quality and well secured environment in which researchers and students can easily and safely rear mosquitoes. This well-equipped insectary is subdivided into 6 sections:

  • A Main room with working area and adults mosquitoes cages;
  • A room for Anopheles larvae rearing (at 27-28°C)
  • A room for Aedes larvae rearing (at 30°C)
  • A special room for susceptible strains rearing
  • A bioassay room where all insecticides testing protocols are run.
  • A room for experimental infections, dissections and microscopy identification.

These rooms’ temperature and hygrometry are monitored by a wireless thermo-humidifier system to ensure appropriate temperature and humidity levels.

Anopheles larvae rearing
Anopheles larvae rearing

Origin of mosquitoes. Field mosquitoes originate from different areas across Cameroon and other African countries. They can be collected at larval stages (from breeding sites) or adult stages (indoor and outdoor aspirations).

In addition, we have insecticide resistant and susceptible laboratory colonies for Anopheles (FANG, FUMOZ, KISUMU, NGOUSSO…) and for Aedes (VCRU, Benin …) which are used as control in our various experiments. And hybrids are also obtained through the crossing of field and lab strains.

Insecticide testing. A special control room is available where insecticide tests are performed.

The different tests carried out at CRID’s insectary include

  • WHO tube assays;
  • Cone assays;
  • CDC bottles assays;
  • Tunnel test.

All tests are done following the latest WHO protocols

Biosecurity. Our insectary is organized in such a way that mosquitoes cannot escape as well as the mosquitoes outside cannot come in.

  •  Boiled water is used to clean trays to enssure larvae and eggs are killed
  • Electric flyswatters are used in case a mosquito escapes cage
  • All doors and windows are protected by anti-mosquito’s net to avoid mosquito escaping from the insectary

January 27, 2020