POST : Post-Doctoral Research Assistant(s) in Transcriptomics, quantitative genetics, Genomics, Functional Genomics
CONTRACT : Full-Time, Fixed-term appointment for up to 36 months subject to meeting project milestones
REPORTS TO : Prof Charles Wondji
ROLE PURPOSE/SUMMARY :
The Centre for Research in Infectious Diseases (CRID) seeks to appoint 5 post-doctoral research assistants to work with Prof Charles Wondji on a Bill and Melinda Gates funded project on the detection of molecular markers for metabolic resistance in malaria vectors. The project focuses on:
- Detecting the genetic loci and variants driving resistance to insecticides in malaria vectors (RNAseq, PoolSeq, target enrichment sequencing),
- Functional validation of the role of candidate genes and genetic variants associated with pyrethroid resistance and design DNA-based field applicable diagnostic tools and validate these in the field (GAL4/UAS transgenic expression in Drosophila, RNAi, metabolism assays with recombinant proteins),
- Design and validation of DNA-based diagnostic assays to detect resistance to insecticides and assessing resistance impact
The project will combine transcriptomic analysis of genes conferring pyrethroid resistance, detection of resistance loci and polymorphisms using targeted enrichment and sequencing method, assessement of the role of complex genomic such as copy number variation, Structural variations in conferring resistance. This will also involve functional characterization of candidate genes and variants, including in vitro heterologous protein expression/analysis of resistance genes, in vivo GAL4-UAS transgenic expression in Drosophila, RNA-interference and bioinformatics. The project will benefit from the collaboration of the Liverpool School of Tropical Medicine and other partners.
The successful candidate must hold a PhD in biological sciences with a solid background either in transcriptomics (RNAseq/qRT-PCR, Gene Ontology etc) quantitative genetics (GWAS), functional genomics or biochemistry or molecular genetics with experience working on insect vectors desirable but not critical. Ability to analyze next-generation sequencing data is also desirable.
These positions will be very suitable for young African scientists abroad who may want to return on the continent to pursue a career in a dynamic local research environment. Successful applicants will also be encouraged and supported to pursue an independent career by applying to personal fellowship or grants building on CRID success in supporting its scientists to obtain various fellowships including Wellcome Trust, FLAIR royal Society, GCRF and NIH
The application must be addressed to firstname.lastname@example.org and should include:
- An updated detailed curriculum vitae;
- A letter of motivation
- Recommendation letter;
Deadline for completed applications: October 31st, 2020.
Shortlisted candidates will be called for an interview on the 09th and 10th November 2020 and the selected one will start the job on December 1st, 2020.